pLEX SC4MOL 519
(Plasmid
#27984)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 27984 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLEX
- Backbone size w/o insert (bp) 11000
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Vector typeMammalian Expression, Lentiviral
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Tetracycline, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Growth instructionsStbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSC4MOL
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SpeciesH. sapiens (human)
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MutationH173Q mutation in SC4MOL
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GenBank IDNM_006745
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Entrez GeneMSMO1 (a.k.a. DESP4, ERG25, MCCPD, SC4MOL)
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Tags
/ Fusion Proteins
- HA (N terminal on insert)
- MYC (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
H173Q caused by 519T->A mutation as described in He et al., J Clin Invest. 2011;121(3):976–984. PMID: 21285510
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLEX SC4MOL 519 was a gift from Igor Astsaturov (Addgene plasmid # 27984 ; http://n2t.net/addgene:27984 ; RRID:Addgene_27984)