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Addgene

pDONR SC4MOL 519
(Plasmid #27981)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27981 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR221
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4762
  • Vector type
    Gateway Donor Vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SC4MOL
  • Species
    H. sapiens (human)
  • Mutation
    H173Q mutation in SC4MOL
  • GenBank ID
    NM_006745
  • Entrez Gene
    MSMO1 (a.k.a. DESP4, ERG25, MCCPD, SC4MOL)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site att1 (unknown if destroyed)
  • 3′ cloning site att2 (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

H173Q caused by 519T->A mutation as described in He et al., J Clin Invest. 2011;121(3):976–984. PMID: 21285510

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR SC4MOL 519 was a gift from Igor Astsaturov (Addgene plasmid # 27981 ; http://n2t.net/addgene:27981 ; RRID:Addgene_27981)
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