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Addgene

pLEX-ccdb Notes

Addgene Diagnostic Digest

This gel depicts the NEB 1kb DNA ladder and a digestion of plasmid 27979 with NheI-HF.

Expected banding pattern:

NheI: If single plasmid: 8.3, 6.3, 2.3 kb. If mixed population: 11.1, 3.4, 2.3 kb.

These results indicate we have a mixed population that contains the correct plasmid and a contaminating DUSP6 plasmid.