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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 27977 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLEX-HA-MYC
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Backbone manufacturerThermo Scientific
- Backbone size w/o insert (bp) 11000
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Vector typeMammalian Expression, Lentiviral
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Tetracycline, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDUSP6
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SpeciesH. sapiens (human)
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MutationC293S: mutation in the phosphatase domain
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Entrez GeneDUSP6 (a.k.a. HH19, MKP3, PYST1)
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Tags
/ Fusion Proteins
- HA (N terminal on insert)
- MYC (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Catalytically inactive DUSP6
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
∆DUSP6 was a gift from Igor Astsaturov (Addgene plasmid # 27977 ; http://n2t.net/addgene:27977 ; RRID:Addgene_27977) -
For your References section:
DUSP6 regulates drug sensitivity by modulating DNA damage response. Bagnyukova TV, Restifo D, Beeharry N, Gabitova L, Li T, Serebriiskii IG, Golemis EA, Astsaturov I. Br J Cancer. 2013 Aug 20;109(4):1063-71. doi: 10.1038/bjc.2013.353. Epub 2013 Jul 9. 10.1038/bjc.2013.353 PubMed 23839489