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Addgene

DUSP6
(Plasmid #27975)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27975 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLEX-MCS
  • Backbone manufacturer
    Thermo Scientific
  • Backbone size w/o insert (bp) 11000
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DUSP6
  • Species
    H. sapiens (human)
  • Entrez Gene
    DUSP6 (a.k.a. HH19, MKP3, PYST1)
  • Promoter CMV
  • Tags / Fusion Proteins
    • HA (N terminal on insert)
    • Myc (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DUSP6 was a gift from Igor Astsaturov (Addgene plasmid # 27975 ; http://n2t.net/addgene:27975 ; RRID:Addgene_27975)
  • For your References section:

    DUSP6 regulates drug sensitivity by modulating DNA damage response. Bagnyukova TV, Restifo D, Beeharry N, Gabitova L, Li T, Serebriiskii IG, Golemis EA, Astsaturov I. Br J Cancer. 2013 Aug 20;109(4):1063-71. doi: 10.1038/bjc.2013.353. Epub 2013 Jul 9. 10.1038/bjc.2013.353 PubMed 23839489