Flag-cIAP2/pRK5
(Plasmid #27973)

• Depositing Lab: Xiaolu Yang
• Publication: Hu et al J Clin Invest. 2006 Jan . 116(1):174-81.

Backbone

• Vector backbone: pRK5
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CIAP2
• Species: H. sapiens (human)
• Entrez Gene: BIRC3 (a.k.a. AIP1, API2, CIAP2, HAIP1, HIAP1, IAP-1, MALT2, MIHC, RNF49, c-IAP2)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid has also been used and described in the following publication: Cellular inhibitor of apoptosis 1 and 2 are ubiquitin ligases for the apoptosis inducer Smac/DIABLO. Hu et al (J Biol Chem. 2003 Mar 21;278(12):10055-60. Epub 2003 Jan 13. https://www.ncbi.nlm.nih.gov/pubmed/12525502 )