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pUT1321-HQ858823
(Plasmid #27905)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27905 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pENTR/D
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2580
  • Vector type
    Entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    E. coli Top10 (Invitrogen)
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    C2H2 transcription factor encoded by Os03g0437200 LOC_Os03g32230
  • Species
    Oryza sativa (rice)
  • Insert Size (bp)
    660
  • GenBank ID
    HQ858823

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This CDS was amplified using PCR from a full length cDNA template from the OSIIEa, OSIIEb, and OSJNEa thru OSJNEf full length cDNA libraries generated by the Guoliang Wang lab at Ohio State University. The PCR product omitted the stop codon. The PCR product was cloned into a Gateway pENTR/D entry vector.

Further information on this gene may be obtained at www.grassius.org using the Genbank number. (The second half of the plasmid name is the Genbank Number for the sequence of the insert)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUT1321-HQ858823 was a gift from John Gray & Erich Grotewold (Addgene plasmid # 27905 ; http://n2t.net/addgene:27905 ; RRID:Addgene_27905)

  • For your References section:

    GRASSIUS: a platform for comparative regulatory genomics across the grasses. Yilmaz A, Nishiyama MY Jr, Fuentes BG, Souza GM, Janies D, Gray J, Grotewold E. Plant Physiol. 2009 Jan;149(1):171-80. doi: 10.1104/pp.108.128579. Epub 2008 Nov 5. 10.1104/pp.108.128579 PubMed 18987217
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