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pUT1453-HQ858747
(Plasmid #27829)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27829 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pENTR/SD
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2601
  • Vector type
    Entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    E. coli TOP10
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PseudoARR-B transcription factor from DR826352
  • Species
    Zea mays
  • Insert Size (bp)
    1545
  • GenBank ID
    HQ858747

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This CDS was amplified using PCR from a full length cDNA template from the Maize Full-length cDNA Project (www.maizecdna.org) and the PCR product included the stop codon. The PCR product was cloned into a Gateway pENTR/SD vector.

Further information on this gene may be obtained at www.grassius.org using the GenBank number. (The second half of the plasmid name is the GenBank Number for the sequence of the insert)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUT1453-HQ858747 was a gift from John Gray & Erich Grotewold (Addgene plasmid # 27829 ; http://n2t.net/addgene:27829 ; RRID:Addgene_27829)

  • For your References section:

    The Maize TFome--development of a transcription factor open reading frame collection for functional genomics. Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E. Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 Aug 26. 10.1111/tpj.12623 PubMed 25053252
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