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Addgene

C17A
(Plasmid #27801)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27801 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP C1
  • Backbone manufacturer
    Clonetech
  • Backbone size w/o insert (bp) 3984
  • Vector type
    Mammalian Expression ; FRET donor control
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cerulean-17-Amber
  • Alt name
    C17A
  • Species
    GFP Varient
  • Insert Size (bp)
    1493
  • Tags / Fusion Proteins
    • Cerulean (N terminal on insert)
    • Amber (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nhe 1 (not destroyed)
  • 3′ cloning site Bam HI (not destroyed)
  • 5′ sequencing primer CCAAAATCAACGGGACTTTCC
  • 3′ sequencing primer CAGGTTCAGGGGGAGGTGTGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Control for fluorescence lifetime measurements (FLIM)

This construct contains Cerulean attached via a 17 amino acid linker to Amber. Amber was generated by mutating Tyrosine 67 in Venus to Cysteine.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    C17A was a gift from Steven Vogel (Addgene plasmid # 27801 ; http://n2t.net/addgene:27801 ; RRID:Addgene_27801)

  • For your References section:

    Cerulean, Venus, and VenusY67C FRET reference standards. Koushik SV, Chen H, Thaler C, Puhl HL, Vogel SS. Biophys J. 2006 Dec 15. 91(12):L99-L101. 10.1529/biophysj.106.096206 PubMed 17040988
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