CAV1-D67G-HA
(Plasmid
#27706)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 27706 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-N1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 3961
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecaveolin-1, mutant D67G
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Alt nameCAV1, cav-1
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SpeciesCanis lupus familiaris
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Insert Size (bp)603
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Mutationmutant D67G
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GenBank IDNM_001003296
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Entrez GeneCAV1
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Tag
/ Fusion Protein
- HA (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CMV-FW (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The point mutation D67G in the DXE motif delays export from the ER.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CAV1-D67G-HA was a gift from Ari Helenius (Addgene plasmid # 27706 ; http://n2t.net/addgene:27706 ; RRID:Addgene_27706) -
For your References section:
Biogenesis of caveolae: stepwise assembly of large caveolin and cavin complexes. Hayer A, Stoeber M, Bissig C, Helenius A. Traffic. 2010 Mar . 11(3):361-82. 10.1111/j.1600-0854.2009.01023.x PubMed 20070607
