APPL1-pmCherryC1
(Plasmid #27683)

• Depositing Lab: Christien Merrifield
• Publication: Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Backbone

• Vector backbone: pmCherry-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4722
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: APPL1
• Species: M. musculus (mouse)
• Insert Size (bp): 2100
• Entrez Gene: Appl1 (a.k.a. 2900057D21Rik, 7330406P05Rik, DIP13)
• Tag / Fusion Protein:
  • mCherry (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: XmaI (not destroyed)
• 5′ sequencing primer: mCherry-F 5'-ccccgtaatgcagaagaaga
• 3′ sequencing primer: SV40pA-R

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Cloned from IMAGE clone: 6511401 (MGC:69973).