pCMV-myc-ASPM MTB
(Plasmid #27668)

• Depositing Lab: Joseph Loturco
• Publication: Paramasivam et al Cell Cycle. 2007 Jul 1. 6(13):1605-12.

Backbone

• Vector backbone: pCMV-Myc
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3800
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Abnormal spindle-like microcephaly associated
• Alt name: ASPM Microtubule Binding Domain
• Species: R. norvegicus (rat)
• Insert Size (bp): 2820
• Mutation: Contains amino‑terminal putative microtubule binding domain of ASPM (amino acids 1‑940)
• Entrez Gene: Aspm (a.k.a. Calmbp1)
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that though this plasmid is in the pCMV-myc backbone, the myc tag is NOT in frame with the insert.