pBABEpuro U3-hTR-500
(Plasmid #27666)

• Depositing Lab: Kathleen Collins
• Publication: Wong et al Genes Dev. 2006 Oct 15. 20(20):2848-58.

Backbone

• Vector backbone: pBABEpuro
• Backbone size w/o insert (bp): 5200
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: U3-hTR-500
• Alt name: human telomerase RNA
• Alt name: TER
• Alt name: TERC
• Species: H. sapiens (human)
• Insert Size (bp): 1300
• Entrez Gene: TERC (a.k.a. DKCA1, PFBMFT2, SCARNA19, TER, TR, TRC3, hTR)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (destroyed during cloning)
• 3′ cloning site: NheI (destroyed during cloning)
• 5′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

sequence is for the insert of human U3 snoRNA promoter driving expression of human telomerase RNA mature sequence (hTR) with 500 bp downstream genomic region included; see Fu and Collins (2003)