pBABEpuroUThTERT+U3-hTR-500
(Plasmid #27665)

• Depositing Lab: Kathleen Collins
• Publication: Wong et al Genes Dev. 2006 Oct 15. 20(20):2848-58.

Backbone

• Vector backbone: pBABEpuro
• Backbone size w/o insert (bp): 5300
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: UThTERT+U3-hTR-500
• Alt name: human telomerase RNA
• Alt name: TER
• Alt name: TERC
• Species: H. sapiens (human)
• Insert Size (bp): 1300
• Entrez Gene: TERC (a.k.a. DKCA1, PFBMFT2, SCARNA19, TER, TR, TRC3, hTR)
• Entrez Gene: TERT (a.k.a. CMM9, DKCA2, DKCB4, EST2, PFBMFT1, TCS1, TP2, TRT, hEST2, hTRT)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SnaB1 (destroyed during cloning)
• 3′ cloning site: SalI (destroyed during cloning)
• 5′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

untagged (UT) human TERT (hTERT) into pBABEpuro U3-hTR-500 (U3 promoter + hTR sequence given as insert; second insert of hTERT sequence available from GenBank); this plasmid is for coexpression of hTERT and hTR