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Addgene

p2RZ
(Plasmid #27664)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27664 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pUC18
  • Backbone size (bp) 2776
  • Vector type
    RNA Transcription

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Alt name
    HDV Ribozyme
  • Tag / Fusion Protein
    • HDV Ribozyme (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer M13 For
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p2RZ was a gift from Graeme Conn (Addgene plasmid # 27664 ; http://n2t.net/addgene:27664 ; RRID:Addgene_27664)
  • For your References section:

    General plasmids for producing RNA in vitro transcripts with homogeneous ends. Walker SC, Avis JM, Conn GL. Nucleic Acids Res. 2003 Aug 1. 31(15):e82. 10.1093/nar/gng082 PubMed 12888534