pET15p65
(Plasmid #27661)

Depositing Lab

Kathleen Collins
Publication

Prathapam et al Nat Struct Mol Biol. 2005 Mar . 12(3):252-7. ( How to cite )
Sequence Information
- Sequences (2)

Full plasmid sequence is not available for this item.

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Ordering

This material is available to academics and nonprofits only.

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	27661	Standard format: Plasmid sent in bacteria as agar stab	1	$85	Add to Cart

Backbone

Vector backbone
pET15
Backbone size w/o insert (bp) 5700
Vector type
Bacterial Expression

Growth in Bacteria

Bacterial Resistance(s)
Ampicillin, 100 μg/mL
Growth Temperature
37°C
Growth Strain(s)
DH5alpha
Copy number
Unknown

Gene/Insert

Gene/Insert name
Tetrahymena telomerase holoenzyme p65
Species
synthetic gene
Insert Size (bp)
1630
Tag / Fusion Protein
- His (N terminal on backbone)

Cloning Information

Cloning method Restriction Enzyme
5′ cloning site NdeI (not destroyed)
3′ cloning site BamHI (not destroyed)
5′ sequencing primer T7 Fwd

(Common Sequencing Primers)

Terms and Licenses

Academic/Nonprofit Terms
- UBMTA
Industry Terms
- Not Available to Industry

Trademarks:

Zeocin® is an InvivoGen trademark.

Depositor Comments

see also O'Connor and Collins (2006)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

For your Materials & Methods section:

pET15p65 was a gift from Kathleen Collins (Addgene plasmid # 27661 ; http://n2t.net/addgene:27661 ; RRID:Addgene_27661)
For your References section:

A telomerase holoenzyme protein enhances telomerase RNA assembly with telomerase reverse transcriptase. Prathapam R, Witkin KL, O'Connor CM, Collins K. Nat Struct Mol Biol. 2005 Mar . 12(3):252-7. 10.1038/nsmb900 PubMed 15696174