pCITETtTERT
(Plasmid
#27660)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 27660 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCITE4a
- Backbone size w/o insert (bp) 3700
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Vector typereticulocyte lysate
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTetrahymena TERT
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Alt namep133
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Speciessynthetic gene
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Insert Size (bp)3360
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCITETtTERT was a gift from Kathleen Collins (Addgene plasmid # 27660 ; http://n2t.net/addgene:27660 ; RRID:Addgene_27660) -
For your References section:
A telomerase holoenzyme protein enhances telomerase RNA assembly with telomerase reverse transcriptase. Prathapam R, Witkin KL, O'Connor CM, Collins K. Nat Struct Mol Biol. 2005 Mar . 12(3):252-7. 10.1038/nsmb900 PubMed 15696174