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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 27653 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUHD10-3
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Backbone manufacturerM. Gossen et al., 1992 (PNAS)
- Backbone size w/o insert (bp) 3150
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCdk1
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SpeciesH. sapiens (human)
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MutationD146N (Dominant Negative)
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Entrez GeneCDK1 (a.k.a. CDC2, CDC28A, P34CDC2)
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Tag
/ Fusion Protein
- HA (YDVPDYA) (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer LNCX
- 3′ sequencing primer EBV_Rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See attachment for description of backbone.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUHD Cdk1 DN HA was a gift from Greg Enders (Addgene plasmid # 27653 ; http://n2t.net/addgene:27653 ; RRID:Addgene_27653)