pUHD Cdk1 WT HA
(Plasmid #27652)

• Depositing Lab: Greg Enders
• Publication: Greg Enders Lab Plasmids (unpublished)

Backbone

• Vector backbone: pUHD10-3
• Backbone manufacturer: M. Gossen et al., 1992 (PNAS)
• Backbone size w/o insert (bp): 3150
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cdk1
• Species: H. sapiens (human)
• Entrez Gene: CDK1 (a.k.a. CDC2, CDC28A, P34CDC2)
• Tag / Fusion Protein:
  • HA (YDVPDYA) (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: LNCX
• 3′ sequencing primer: EBV_Rev

Resource Information

• Supplemental Documents:
  • pUHD data
• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See attachment for description of backbone.