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Addgene

pcdna3 flag - ULK2 wt
(Plasmid #27637)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27637 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mouse ULK2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3108
  • Mutation
    Initial M removed from ULK2.
  • Entrez Gene
    Ulk2 (a.k.a. RP23-278F12.5, A830085I22Rik, AU015340, Unc51.2, mKIAA0623)
  • Tag / Fusion Protein
    • flag (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site none/attR1 (unknown if destroyed)
  • 3′ cloning site none/attR2 (unknown if destroyed)
  • 5′ sequencing primer t7 promotor
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcdna3 flag - ULK2 wt was a gift from Reuben Shaw (Addgene plasmid # 27637 ; http://n2t.net/addgene:27637 ; RRID:Addgene_27637)

  • For your References section:

    Phosphorylation of ULK1 (hATG1) by AMP-Activated Protein Kinase Connects Energy Sensing to Mitophagy. Egan DF, Shackelford DB, Mihaylova MM, Gelino SR, Kohnz RA, Mair W, Vasquez DS, Joshi A, Gwinn DM, Taylor R, Asara JM, Fitzpatrick J, Dillin A, Viollet B, Kundu M, Hansen M, Shaw RJ. Science. 2010 Dec 23. ():. 10.1126/science.1196371 PubMed 21205641
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