pcdna3 flag - ULK1 wt
(Plasmid #27636)

• Depositing Lab: Reuben Shaw
• Publication: Egan et al Science. 2010 Dec 23. ():.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mouse ULK1
• Species: M. musculus (mouse)
• Insert Size (bp): 3150
• Mutation: Initial M from ULK1 removed.
• GenBank ID: NP_033495.2
• Entrez Gene: Ulk1 (a.k.a. AU041434, Unc51.1, mKIAA0722)
• Tag / Fusion Protein:
  • flag (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ cloning site: none/attR1 (unknown if destroyed)
• 3′ cloning site: none/attR2 (unknown if destroyed)
• 5′ sequencing primer: t7 promotor

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry