pEBG‐AMPK
α1(1‐312)
(Plasmid #27632)

• Depositing Lab: Reuben Shaw
• Publication: Egan et al Science. 2010 Dec 23. ():.

Backbone

• Vector backbone: pEBG
• Backbone size w/o insert (bp): 6100
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: AMPK
α1(1‐312)
• Species: R. norvegicus (rat)
• Insert Size (bp): 2400
• Mutation: Constitutively
 active
 by 
truncation 
after 
amino 
acid 
312.
• Entrez Gene: Prkaa1
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: EF-1a Forward

Resource Information

• Articles Citing this Plasmid:
  • 11 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid was first described in the following article: Crute et al. Functional domains of the alpha1 catalytic subunit of the AMP-activated protein kinase. J Biol Chem (1998) vol. 273 (52) pp. 35347-54.