Skip to main content
Addgene

pcdna6.2- myc ULK1 k46I
(Plasmid #27630)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27630 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pcDNA 6.2
 V5
 dest
 (Invitrogen)
  • Backbone size w/o insert (bp) 7341
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mouse myc ULK1 K46I
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3192
  • Mutation
    Initial M from ULK1 removed. Silent mutation at Arginine 5. Does not affect amino acid sequence.K46I mutation makes kinase catalytically inactive.
  • Entrez Gene
    Myc (a.k.a. Myc2, Niard, Nird, bHLHe39)
  • Tag / Fusion Protein
    • myc (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site none/attR1 (unknown if destroyed)
  • 3′ cloning site none/attR2 (unknown if destroyed)
  • 5′ sequencing primer t7 promotor
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

C-term V5 tag not expressed because stop codon at end of ULK1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcdna6.2- myc ULK1 k46I was a gift from Reuben Shaw (Addgene plasmid # 27630 ; http://n2t.net/addgene:27630 ; RRID:Addgene_27630)

  • For your References section:

    Phosphorylation of ULK1 (hATG1) by AMP-Activated Protein Kinase Connects Energy Sensing to Mitophagy. Egan DF, Shackelford DB, Mihaylova MM, Gelino SR, Kohnz RA, Mair W, Vasquez DS, Joshi A, Gwinn DM, Taylor R, Asara JM, Fitzpatrick J, Dillin A, Viollet B, Kundu M, Hansen M, Shaw RJ. Science. 2010 Dec 23. ():. 10.1126/science.1196371 PubMed 21205641
Related items:
Commonly requested with: