pcdna6.2- myc ULK1 wt
(Plasmid #27629)

• Depositing Lab: Reuben Shaw
• Publication: Egan et al Science. 2010 Dec 23. ():.

Backbone

• Vector backbone: pcDNA 6.2
 V5
 dest
 (Invitrogen)
• Backbone size w/o insert (bp): 7341
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mouse myc ULK1
• Species: M. musculus (mouse)
• Insert Size (bp): 3192
• Mutation: Initial M from ULK1 removed. Silent mutation at Arginine 5. Does not affect amino acid sequence.
• Entrez Gene: Myc (a.k.a. Myc2, Niard, Nird, bHLHe39)
• Tag / Fusion Protein:
  • myc (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ cloning site: none/attR1 (unknown if destroyed)
• 3′ cloning site: none/attR2 (unknown if destroyed)
• 5′ sequencing primer: t7 promotor

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

C-term V5 not expressed because of stop codon at end of ULK1.