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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 27569 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep156RRL-sinPPT-CMV-GFP-PRE/Nhe I
- Backbone size w/o insert (bp) 8085
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Vector typeMammalian Expression, Lentiviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Growth instructionsStbl3, 37oC
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEnhance Green Fluorescent Protein
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Alt nameeGFP
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Insert Size (bp)719
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMVtight
- 3′ sequencing primer WPRErev (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe CMVTRE3G promoter was a gift from Dr. Dominic Esposito (SAIC Frederick). Tet-On 3G compatible plasmid.
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
There are a few mismatches between Addgene's quality control sequence and depositor's reference sequence. However the changes are in vector sequence only, features unaffected. Depositor tested this construct and it was functional.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti CMVTRE3G eGFP Neo (w821-1) was a gift from Eric Campeau (Addgene plasmid # 27569 ; http://n2t.net/addgene:27569 ; RRID:Addgene_27569)