pLenti CMVTRE3G Puro DEST (w811-1)
(Plasmid #27565)

• Purpose: (Empty Backbone) 3rd gen lentiviral Gateway destination vector. Inserted genes are expressed only in the resence of rtTA3.
• Depositing Lab: Eric Campeau
• Publication: other plasmids (unpublished)

Backbone

• Vector backbone: p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
• Backbone size (bp): 9442
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Growth instructions: DB3.1, 37oC
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Empty

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMVtight
• 3′ sequencing primer: WPRErev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The CMVTRE3G promoter was a gift from Dr. Dominic Esposito (SAIC Frederick). Tet-On 3G compatible plasmid.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Tet Systems Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There are a few mismatches between Addgene's quality control sequence and depositor's reference sequence. However the changes are in vector sequence only, features unaffected. Depositor tested this construct and it was functional.