p2.1 (pAGL2A)
(Plasmid
#27563)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 27563 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGL2-Promoter
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 5789
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameENO1 promoter
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SpeciesH. sapiens (human)
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Insert Size (bp)68
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Entrez GeneENO1 (a.k.a. ENO1-IT1, ENO1L1, HEL-S-17, MPB1, NNE, PPH)
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Tag
/ Fusion Protein
- Luciferase (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer LucNRev (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
68-bp sequence from the ENO1 promoter (nt -416 to -347).
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p2.1 (pAGL2A) was a gift from Gregg Semenza (Addgene plasmid # 27563 ; http://n2t.net/addgene:27563 ; RRID:Addgene_27563) -
For your References section:
Hypoxia response elements in the aldolase A, enolase 1, and lactate dehydrogenase A gene promoters contain essential binding sites for hypoxia-inducible factor 1. Semenza GL, Jiang BH, Leung SW, Passantino R, Concordet JP, Maire P, Giallongo A. J Biol Chem. 1996 Dec 20. 271(51):32529-37. 10.1074/jbc.271.51.32529 PubMed 8955077