pCLXSN-GFP
(Plasmid #27557)

• Purpose: (Empty Backbone)
• Depositing Lab: Shao-Cong Sun
• Publication: Reiley et al Mol Cell Biol. 2005 May . 25(10):3886-95.

Backbone

• Vector backbone: pCLXSN
• Backbone manufacturer: Addgene Plasmid 12343 (Dr. Inder Verma)
• Backbone size (bp): 7807
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pLXSN 5'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: PCLXSN-based plasmids were constructed using the pCLXSN vector obtained from Dr. Inder Verma.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Here, the pCLXSN vector was modified by replacing its neomycin-resistant gene with the green fluorescence protein (GFP) gene.