pCLXSN-HA-CYLD
(Plasmid #27556)

• Depositing Lab: Shao-Cong Sun
• Publication: Reiley et al Mol Cell Biol. 2005 May . 25(10):3886-95.

Backbone

• Vector backbone: pCLXSN
• Backbone manufacturer: Addgene Plasmid 12343 (Dr. Inder Verma)
• Backbone size w/o insert (bp): 7807
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CYLD
• Species: H. sapiens (human)
• Mutation: R156G and G179R
• Entrez Gene: CYLD (a.k.a. BRSS, CDMT, CYLD1, CYLDI, EAC, FTDALS8, MFT, MFT1, SBS, TEM, USPL2)
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pLXSN 5'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: PCLXSN-based plasmids were constructed using the pCLXSN vector obtained from Dr. Inder Verma (do not remember if there is MTA)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that this is the exact plasmid (including mutations) used in the associated publication listed below.