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pCMV4-NIK-HA
(Plasmid #27554)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27554 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV4
  • Backbone manufacturer
    Dr. David Russell, UTSW
  • Backbone size w/o insert (bp) 4874
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NIK
  • Species
    H. sapiens (human)
  • Mutation
    NIK starts at amino acid 3; C52R and R139H mutations when compared to NP_003945.2
  • Entrez Gene
    MAP3K14 (a.k.a. FTDCR1B, HS, HSNIK, IMD112, NIK)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site EcoRV (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer hGH-pA-R
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    To create pCMV4-HA-NIK, we PCR amplified the NIK cDNA using the Flag-NIK plasmid (obtained from Dr. David Wallach with MTA) as template.
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

According to the depositing laboratory, the differences in NIK compared to GenBank reference sequence NP_003945.2 do not affect the function of NIK.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV4-NIK-HA was a gift from Shao-Cong Sun (Addgene plasmid # 27554 ; http://n2t.net/addgene:27554 ; RRID:Addgene_27554)

  • For your References section:

    Negative regulation of the nuclear factor kappa B-inducing kinase by a cis-acting domain. Xiao G, Sun SC. J Biol Chem. 2000 Jul 14. 275(28):21081-5. 10.1074/jbc.M002552200 PubMed 10887201
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