pLPCXU6PT7SK
(Plasmid #27549)

• Depositing Lab: Kathleen Collins
• Publication: Hogg et al RNA. 2007 Jun . 13(6):868-80.

Backbone

• Vector backbone: pLPCX
• Backbone size w/o insert (bp): 6000
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: U6PT7SK
• Alt name: RAT tag
• Alt name: 7SK RNA
• Alt name: U6 snRNA promoter
• Species: H. sapiens (human)
• Insert Size (bp): 700
• Entrez Gene: RN7SK (a.k.a. 7SK)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (destroyed during cloning)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • Coat Protein Purification Protocol
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that there are some slight discrepancies between Addgene's quality control sequence and the sequence provided by the depositor. These differences should not affect function.