pcDNA-HA-CYLD
(Plasmid
#27547)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 27547 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepcDNA
-
Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5400
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameCYLD
-
SpeciesH. sapiens (human)
-
MutationR156G and G179R
-
Entrez GeneCYLD (a.k.a. BRSS, CDMT, CYLD1, CYLDI, EAC, FTDALS8, MFT, MFT1, SBS, TEM, USPL2)
-
Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer BGH-Rev (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that this is the exact plasmid (including mutations) used in the associated publication listed below.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pcDNA-HA-CYLD was a gift from Shao-Cong Sun (Addgene plasmid # 27547 ; http://n2t.net/addgene:27547 ; RRID:Addgene_27547) -
For your References section:
Regulation of the deubiquitinating enzyme CYLD by IkappaB kinase gamma-dependent phosphorylation. Reiley W, Zhang M, Wu X, Granger E, Sun SC. Mol Cell Biol. 2005 May . 25(10):3886-95. 10.1128/MCB.25.10.3886-3895.2005 PubMed 15870263