pBS ZfHoxd3a
(Plasmid
#27534)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 27534 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBluescript
- Backbone size w/o insert (bp) 3000
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Vector typeCloning vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHoxd3a
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Alt name3' end clone
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SpeciesD. rerio (zebrafish)
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Entrez Genehoxd3a (a.k.a. Z-56, hoxd3, im:7144208)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AccI (unknown if destroyed)
- 3′ cloning site PstI? (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene's quality control sequence has some mismatches with NCBI's sequence for this gene; the mismatches should not affect plasmid function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS ZfHoxd3a was a gift from Victoria Prince (Addgene plasmid # 27534 ; http://n2t.net/addgene:27534 ; RRID:Addgene_27534) -
For your References section:
Zebrafish hox genes: expression in the hindbrain region of wild-type and mutants of the segmentation gene, valentino. Prince VE, Moens CB, Kimmel CB, Ho RK. Development. 1998 Feb . 125(3):393-406. PubMed 9425135