pGEM-T ZfHoxb10a
(Plasmid
#27530)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 27530 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEM-T
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHoxb10a
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Alt name3' end clone
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)1000
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Entrez Genehoxb10a (a.k.a. z-122, hoxb10, im:7139959, wu:fc25g03)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRV (destroyed during cloning)
- 3′ cloning site EcoRV (destroyed during cloning)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene's quality control sequence has some mismatches with NCBI's sequence for this gene; the mismatches should not affect plasmid function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEM-T ZfHoxb10a was a gift from Victoria Prince (Addgene plasmid # 27530 ; http://n2t.net/addgene:27530 ; RRID:Addgene_27530) -
For your References section:
Zebrafish hox genes: genomic organization and modified colinear expression patterns in the trunk. Prince VE, Joly L, Ekker M, Ho RK. Development. 1998 Feb . 125(3):407-20. PubMed 9425136