pBS ZfHoxa2b
(Plasmid
#27514)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 27514 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript SK-
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 3000
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Vector typeRiboprobe synthesis
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHoxa2b
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)1100
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Entrez Genehoxa2b (a.k.a. Z-75, hoxa2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site SacII (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS ZfHoxa2b was a gift from Victoria Prince (Addgene plasmid # 27514 ; http://n2t.net/addgene:27514 ; RRID:Addgene_27514)