pPD162.21
(Plasmid
#27511)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 27511 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneN/A
- Backbone size w/o insert (bp) 2500
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namepmyo-3::dsRed::let-858_3'UTR
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SpeciesC. elegans (nematode)
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Insert Size (bp)4000
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPD162.21 was a gift from Andrew Fire (Addgene plasmid # 27511 ; http://n2t.net/addgene:27511 ; RRID:Addgene_27511) -
For your References section:
Cell autonomous specification of temporal identity by Caenorhabditis elegans microRNA lin-4. Zhang H, Fire AZ. Dev Biol. 2010 Aug 15. 344(2):603-10. 10.1016/j.ydbio.2010.05.018 PubMed 20493184
