pPD133.47
(Plasmid #27510)

• Depositing Lab: Andrew Fire
• Publication: Zhang et al Dev Biol. 2010 Aug 15. 344(2):603-10.

Backbone

• Vector backbone: N/A
• Backbone size w/o insert (bp): 2000
• Vector type: Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: pmyo-3::cfp::lacZ::unc-54_3'UTR
• Species: C. elegans (nematode)
• Insert Size (bp): 8000

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: N/A

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that there are some slight discrepancies between Addgene's quality control sequence and the theoretical sequence from the Fire lab. These differences should not affect function.