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Addgene

pHZ146
(Plasmid #27508)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27508 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    n/a
  • Backbone size w/o insert (bp) 4078
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    pREAR::lin-4::unc-54_3'UTR
  • Alt name
    lin-4
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    90
  • Entrez Gene
    lin-4 (a.k.a. CELE_F59G1.6)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that there are some slight discrepancies between Addgene's quality control sequence and the theoretical sequence from the Fire lab. These differences should not affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHZ146 was a gift from Andrew Fire (Addgene plasmid # 27508 ; http://n2t.net/addgene:27508 ; RRID:Addgene_27508)

  • For your References section:

    Cell autonomous specification of temporal identity by Caenorhabditis elegans microRNA lin-4. Zhang H, Fire AZ. Dev Biol. 2010 Aug 15. 344(2):603-10. 10.1016/j.ydbio.2010.05.018 PubMed 20493184
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