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Addgene

pHZ018
(Plasmid #27504)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27504 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3956
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    lin-4
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    695
  • Entrez Gene
    lin-4 (a.k.a. CELE_F59G1.6)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHZ018 was a gift from Andrew Fire (Addgene plasmid # 27504 ; http://n2t.net/addgene:27504 ; RRID:Addgene_27504)
  • For your References section:

    Cell autonomous specification of temporal identity by Caenorhabditis elegans microRNA lin-4. Zhang H, Fire AZ. Dev Biol. 2010 Aug 15. 344(2):603-10. 10.1016/j.ydbio.2010.05.018 PubMed 20493184