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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 27503 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepTS400
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameLasR, LasB::LacZ fusion
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Alt nametranscriptional regulator LasR
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SpeciesPseudomonas aeruginosa
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Insert Size (bp)800
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Entrez GenelasR (a.k.a. PA1430)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Expresses LasR under the control of the lac promoter. Contains a lasB::lacZ reporter of lasB promoter activity
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKDT17 was a gift from Peter Greenberg (Addgene plasmid # 27503 ; http://n2t.net/addgene:27503 ; RRID:Addgene_27503) -
For your References section:
Structure of the autoinducer required for expression of Pseudomonas aeruginosa virulence genes. Pearson JP, Gray KM, Passador L, Tucker KD, Eberhard A, Iglewski BH, Greenberg EP. Proc Natl Acad Sci U S A. 1994 Jan 4. 91(1):197-201. 10.1073/pnas.91.1.197 PubMed 8278364