pOZ-N-FH RAP80
(Plasmid #27501)

• Depositing Lab: Roger Greenberg
• Publication: Patterson-Fortin et al J Biol Chem. 2010 Oct 1. 285(40):30971-81.

Backbone

• Vector backbone: pOZ-N
• Backbone size w/o insert (bp): 7900
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: IL-2 receptor

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: RAP80
• Species: H. sapiens (human)
• Entrez Gene: UIMC1 (a.k.a. RAP80, X2HRIP110)
• Tags / Fusion Proteins:
  • FLAG (N terminal on insert)
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pBMN5'

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There are I41V and E175G mutations in RAP80 that should not affect plasmid function.