pOZ-N-FH PSMD14
(Plasmid
#27500)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 27500 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepOZ-N
- Backbone size w/o insert (bp) 7900
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Vector typeMammalian Expression, Retroviral
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Selectable markersIL-2 receptor
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePSMD14
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SpeciesH. sapiens (human)
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MutationM1V
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Entrez GenePSMD14 (a.k.a. PAD1, POH1, RPN11)
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Tags
/ Fusion Proteins
- FLAG (N terminal on backbone)
- HA (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pBMN5' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pOZ-N-FH PSMD14 was a gift from Roger Greenberg (Addgene plasmid # 27500 ; http://n2t.net/addgene:27500 ; RRID:Addgene_27500) -
For your References section:
Differential regulation of JAMM domain deubiquitinating enzyme activity within the RAP80 complex. Patterson-Fortin J, Shao G, Bretscher H, Messick TE, Greenberg RA. J Biol Chem. 2010 Oct 1. 285(40):30971-81. 10.1074/jbc.M110.135319 PubMed 20656689