Skip to main content
Addgene

pBT239
(Plasmid #27494)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27494 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pBluescript SK(+)
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2800
  • Vector type
    Mouse Targeting

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5alpha Standard LB buffer at 37 degree
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TRE-tdTomato-myc
  • Species
    n/a
  • Insert Size (bp)
    2266
  • Tag / Fusion Protein
    • Myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer M13Reverse
  • 3′ sequencing primer M13Forward
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBT239 was a gift from Liqun Luo (Addgene plasmid # 27494 ; http://n2t.net/addgene:27494 ; RRID:Addgene_27494)

  • For your References section:

    Cortical representations of olfactory input by trans-synaptic tracing. Miyamichi K, Amat F, Moussavi F, Wang C, Wickersham I, Wall NR, Taniguchi H, Tasic B, Huang ZJ, He Z, Callaway EM, Horowitz MA, Luo L. Nature. 2010 Dec 22. ():. 10.1038/nature09714 PubMed 21179085
Related items:
Commonly requested with: