NGFR 210Y177
(Plasmid #27487)

• Depositing Lab: Warren Pear
• Publication: He et al Blood. 2002 Apr 15;99(8):2957-68.

Backbone

• Vector backbone: NGFR
• Backbone manufacturer: Warren Pear Lab
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: bcr/abl (P210)
• Alt name: bcr/abl
• Species: H. sapiens (human)
• Insert Size (bp): 7200
• Mutation: Tyrosine 177 mutated to Phenylalanine (Y177F)
• Entrez Gene: ABL1 (a.k.a. ABL, BCR-ABL, CHDSKM, JTK7, bcr/abl, c-ABL, c-ABL1, p150, v-abl)
• Entrez Gene: BCR (a.k.a. ALL, BCR1, CML, D22S11, D22S662, PHL)
• Tags / Fusion Proteins:
  • IRES (C terminal on insert)
  • NGFR (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pLXSN 5'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry