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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 27486 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneNGFR
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Backbone manufacturerWarren Pear Lab
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namebcr/abl (P210)
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Alt namebcr/abl
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SpeciesH. sapiens (human)
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Insert Size (bp)7200
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MutationContains the "complete" bcr/abl fusion
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Entrez GeneABL1 (a.k.a. ABL, BCR-ABL, CHDSKM, JTK7, bcr/abl, c-ABL, c-ABL1, p150, v-abl)
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Entrez GeneBCR (a.k.a. ALL, BCR1, CML, D22S11, D22S662, PHL)
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Tags
/ Fusion Proteins
- IRES (C terminal on insert)
- NFGR (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pLXSN 5' (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NGFR P210 was a gift from Warren Pear (Addgene plasmid # 27486 ; http://n2t.net/addgene:27486 ; RRID:Addgene_27486) -
For your References section:
The coiled-coil domain and Tyr177 of bcr are required to induce a murine chronic myelogenous leukemia-like disease by bcr/abl. He Y, Wertheim JA, Xu L, Miller JP, Karnell FG, Choi JK, Ren R, Pear WS.. Blood. 2002 Apr 15;99(8):2957-68. 10.1182/blood.V99.8.2957 PubMed 11929787