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Addgene

Mig P190 (Mig 185)
(Plasmid #27483)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27483 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MSCV2.2 (MIG)
  • Backbone manufacturer
    Hawley et al., 1994
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    P190
  • Species
    H. sapiens (human)
  • Mutation
    Contains only exon 1 (e1) of bcr fused to abl
  • Entrez Gene
    ABCA4 (a.k.a. ABC10, ABCR, ARMD2, CORD3, FFM, RMP, RP19, STGD, STGD1)
  • Entrez Gene
    BCR (a.k.a. ALL, BCR1, CML, D22S11, D22S662, PHL)
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Mig P190 (Mig 185) was a gift from Warren Pear (Addgene plasmid # 27483 ; http://n2t.net/addgene:27483 ; RRID:Addgene_27483)

  • For your References section:

    The coiled-coil domain and Tyr177 of bcr are required to induce a murine chronic myelogenous leukemia-like disease by bcr/abl. He Y, Wertheim JA, Xu L, Miller JP, Karnell FG, Choi JK, Ren R, Pear WS.. Blood. 2002 Apr 15;99(8):2957-68. 10.1182/blood.V99.8.2957 PubMed 11929787
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