pFlag-CMV2-WBP1
(Plasmid #27477)

• Depositing Lab: Marius Sudol
• Publication: Chen et al J Biol Chem. 1997 Jul 4. 272(27):17070-7.

Backbone

• Vector backbone: pFlag CMV2
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: WW domain binding protein-1
• Alt name: WBP-1
• Species: H. sapiens (human)
• Insert Size (bp): 940
• GenBank ID: NM_012477
• Entrez Gene: WBP1 (a.k.a. WBP-1)
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (unknown if destroyed)
• 3′ cloning site: Kpn1 (unknown if destroyed)
• 5′ sequencing primer: CMV

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry