pFlag-CMV2-WBP1
(Plasmid
#27477)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 27477 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFlag CMV2
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameWW domain binding protein-1
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Alt nameWBP-1
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SpeciesH. sapiens (human)
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Insert Size (bp)940
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GenBank IDNM_012477
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Entrez GeneWBP1 (a.k.a. WBP-1)
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Tag
/ Fusion Protein
- Flag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (unknown if destroyed)
- 3′ cloning site Kpn1 (unknown if destroyed)
- 5′ sequencing primer CMV (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFlag-CMV2-WBP1 was a gift from Marius Sudol (Addgene plasmid # 27477 ; http://n2t.net/addgene:27477 ; RRID:Addgene_27477) -
For your References section:
Characterization of the WW domain of human yes-associated protein and its polyproline-containing ligands. Chen HI, Einbond A, Kwak SJ, Linn H, Koepf E, Peterson S, Kelly JW, Sudol M. J Biol Chem. 1997 Jul 4. 272(27):17070-7. 10.1074/jbc.272.27.17070 PubMed 9202023