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Addgene

pBABEhygro-Flag-ZO-2-1st-PDZ-mut
(Plasmid #27474)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27474 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABE-hygro
  • Backbone manufacturer
    Addgene
  • Backbone size w/o insert (bp) 5558
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Zonula Occludens 2
  • Alt name
    ZO-2
  • Alt name
    TJP2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3573
  • Mutation
    K38E, F44H, I46E and V48E
  • GenBank ID
    AAH27592
  • Entrez Gene
    TJP2 (a.k.a. C9DUPq21.11, DFNA51, DUP9q21.11, FHCA1, PFIC4, X104, ZO2)
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)
  • 5′ sequencing primer pBABE 5 primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABEhygro-Flag-ZO-2-1st-PDZ-mut was a gift from Marius Sudol (Addgene plasmid # 27474 ; http://n2t.net/addgene:27474 ; RRID:Addgene_27474)

  • For your References section:

    Functional complexes between YAP2 and ZO-2 are PDZ domain-dependent, and regulate YAP2 nuclear localization and signalling. Oka T, Remue E, Meerschaert K, Vanloo B, Boucherie C, Gfeller D, Bader GD, Sidhu SS, Vandekerckhove J, Gettemans J, Sudol M. Biochem J. 2010 Nov 25. 432(3):461-72. 10.1042/BJ20100870 PubMed 20868367
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