pRS303 Gal TDP-43 YFP
(Plasmid
#27468)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 27468 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRS303 Gal
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTDP-43
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Alt nameTDP43
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SpeciesH. sapiens (human)
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MutationD23A mutation in TDP-43 sequence
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Entrez GeneTARDBP (a.k.a. ALS10, TDP-43)
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Tag
/ Fusion Protein
- YFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13-Fwd
- 3′ sequencing primer M13-Rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRS303 Gal TDP-43 YFP was a gift from Aaron Gitler (Addgene plasmid # 27468 ; http://n2t.net/addgene:27468 ; RRID:Addgene_27468) -
For your References section:
A yeast TDP-43 proteinopathy model: Exploring the molecular determinants of TDP-43 aggregation and cellular toxicity. Johnson BS, McCaffery JM, Lindquist S, Gitler AD. Proc Natl Acad Sci U S A. 2008 Apr 29. 105(17):6439-44. 10.1073/pnas.0802082105 PubMed 18434538