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Addgene

pRS416 Gal TDP43 M337V
(Plasmid #27460)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27460 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS416GAL
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TDP-43
  • Species
    H. sapiens (human)
  • Mutation
    Methionine 337 mutated to Valine (M337V)
  • Entrez Gene
    TARDBP (a.k.a. ALS10, TDP-43)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer pRSGal-Fwd GTTAATATACCTCTATACTTTAACGTCAAGGAGA
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS416 Gal TDP43 M337V was a gift from Aaron Gitler (Addgene plasmid # 27460 ; http://n2t.net/addgene:27460 ; RRID:Addgene_27460)

  • For your References section:

    TDP-43 is intrinsically aggregation-prone, and amyotrophic lateral sclerosis-linked mutations accelerate aggregation and increase toxicity. Johnson BS, Snead D, Lee JJ, McCaffery JM, Shorter J, Gitler AD. J Biol Chem. 2009 Jul 24. 284(30):20329-39. 10.1074/jbc.M109.010264 PubMed 19465477
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